Screening of novel antifungal agents through the morphological change in rice blast fungi.


Upon exposure to one drug, rice blast fungus Magnaporthe oryzae often dynamically and specifically changes their shape depending on the mode of action of the drug. It prompted us to construct the encyclopedia of fungus cell morphology, consisting of the unique cell-shape changes induced by various compounds, including well-defined antifungal agents. Particularly, we have examined the effect of 200 routinely used compounds in a time- and dose-dependent manner and investigated the relationship between the phenotypes and the action mechanism of drugs. Now, we could easily discriminate the phenotypes induced by well-known antifungals, such as polyoxins (chitin synthesis; swelling), azoles (ergosterol synthesis; toxic) and griseofulvin (tubulin; short). Our screening system allows us to classify test compounds to classes of compounds sharing similar mechanism of action and to identify antifungal compounds with novel mechanism of action effectively.

作用既知薬剤:Polyoxin A

control Polyoxin A
Polyoxin A, a chitin synthesis inhibitor, induces characteristic swelling in M.oryzae.

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