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Cylolrienin A activates a kinase with an apparent molecular mass of 36 kDa with kinetics different from JNK/SAPK and p38 MAPK in HL-60 cells.
(A) in-gel kinase activity of cells treated with cytotrienin A. Cells were incubated with cytotrienin A at the concentrations indicated. Cell lysates were eleclrophoresed in 10% SDS-PAGE, and kinase activity was determined by an in-gel kinase assay using MBP as a substrate. (B) kinetics of the activation of p.36 MBP kinase. JNK/SAPK. and p.38 MAPK during apoptosis induced by cytotrienin A. Cells were incubated with 100 ng/ml cytotrienin A for the times indicated, and the activation of the JNK/SAPK and p38 MAPK was analyzed by Western blotting using antibodies that specifically recognize the activated JNK/SAPK and p38 MAPK kinases. The p36 MBP kinase activity was measured as described in A. (C) effect of a specific inhibitor of p38 MAPK, SB203580, on p36 MBP kinase activation. Cells were treated with 100 ng/ml cytotrienin A in the presence of SB203580. After incubating for 2 h, p38 MAPK activation and p36 MBP kinase activity were measured as described in B. (Ref:1)


  1. H. Kakeya, R. Onose, H. Osada.
    Caspase-mediated activation of a 36-kDa myelin basic protein kinase during anticancer drug-induced apoptosis.
    Cancer Res., 58, 4888- 4894 (1998).

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