Lopez Julius Adam Velasco


Lopez Julius Adam Velasco

RESEARCH INTEREST

I am fascinated with the natural products chemistry of various sources. I have worked on plants, marine bacteria, and cyanobacteria. Currently, I am working on soil bacteria and fungi. I enjoy devising strategies to isolate and purify compounds from complex extracts, and elucidating the structure of new compounds. It is my hope that my efforts would lead to the development of drug candidates and a better understanding of biosynthetic pathways and bioactivity mechanisms.

EDUCATION

2013-2016 Ph.D., Hokkaido University
2008-2012 M.S. Chemistry, University of the Philippines-Diliman
2004-2007 B.S. Chemistry, University of the Philippines-Diliman

WORK HISTORY

2017-present Postdoctoral Researcher, Chemical Biology Research Group, RIKEN
2017 Postdoctoral Researcher, Hokkaido University

PUBLICATIONS

  1. Lopez JAV, Nogawa T, Futamura Y, Aono H, Hashizume D, Osada H.: N-Acetyl-α-hydroxy-β-oxotryptamine, a racemic natural product isolated from Streptomyces sp. 80H647.
    J Antibiot, 74: 477–479 (2021) PMID: 33879862 [ doi: 10.1038/s41429-021-00420-7 ]
  2. Lopez JAV and Osada H.
    Fraction libraries and genetics as tools for natural product research: focus on decalin-containing compounds. In: Liu HB and Begley TP (eds.) Comprehensive natural products III: chemistry and biology. vol. 7, pp. 375-409, Elsevier, (2020) [ doi: 10.1016/B978-0-12-409547-2.14658-X]
  3. Lopez JAV, Nogawa T, Futamura Y, Shimizu T, Osada H.: Nocardamin glucuronide, a new member of the ferrioxamine siderophores isolated from the ascamycin-producing strain Streptomyces sp. 80H647.
    J Antibiot, 72(12): 991-995 (2019) PMID: 31395970 [ doi: 10.1038/s41429-019-0217-5 ]
  4. LOPEZ, J. A. V.; Vairappan, C. S.; Umezawa, T.; Matsuda, F.; Okino, T. Columbamides D and E: Chlorinated Fatty Acid Amides from Marine Cyanobacterium Moorea bouillonii Collected in Malaysia.
    Org Lett 19, 4231-4234 (2017)

  5. Petitbois, J. G.; Casalme, L. O.; LOPEZ, J. A. V.; Alarif, W. M.; Abdel-Lateff, A.; Al-Lihaibi, S. S.; Yoshimura, E.; Nogata, Y.; Umezawa, T.; Matsuda, F.; Okino, T. Serinolamides and Lyngbyabellins from an Okeania sp. Cyanobacterium Collected from the Red Sea.
    J Nat Prod 80, 2708–2715 (2017)

  6. LOPEZ, J. A. V.; Al-Lihaibi, S. S.; Alarif, W. M.; Abdel-Lateff, A.; Nogata, Y.; Washio, K.; Morikawa, M.; Okino, T. Wewakazole B, a Cytotoxic Cyanobactin from the Cyanobacterium Moorea producens Collected in the Red Sea.
    J Nat Prod 79, 1213–1218 (2016)

Suresh PANTHEE


Suresh PANTHEE
(Ph.D, Foreign Postdoctoral Researcher)

INTEREST OF MY RESEARCH

I am searching for biomediators of reveromycin biosynthesis.

EDUCATION

2001- 2005Institute of Medicine, Tribhuvan University, Nepal 
2006- 2008 Department of Pharmacy, Kathmandu University, Nepal  

DEGREES

BPharm (2005)Tribhuvan University, Kathmandu, Nepal 
MPharm (2008)Kathmandu University, Dhulikhel, Nepal 

APPOINTMENTS

2005 - 2008 Asst. Lecturer, Nobel College of Medical Sciences 
2007 - 2008 Chairperson of Pharmacy Education, International Pharmaceutical Students' Federation (IPSF) 
2008 - 2015Antibiotics Laboratory, RIKEN
2015.Apr.-RIKEN Center for Sustainable Resource Science - Chemical Biology Research Group

MY HOBBY

Reading Books

  
PUBLICATIONS

Panthee, S., Lohani, S. P.
In-vitro adsorption studies of paracetamol to activated charcoal capsule, powder and suspension.
The Open Toxicology Journal, 2, 22-25,(2008).

Tatsuro KAWAMURA


Tatsuro KAWAMURA (Ph.D.)

INTEREST OF MY RESEARCH

My research interest is in the diversity of the molecular mechanisms underlying the redox regulation and metabolism in cancer cells. For better understanding of the cellular redox regulation systems and application to disease treatment, I have been working on identification and characterization of small-molecule redox modulators.

EDUCATION

2001-2005 Keio University, Department of Science and Technology
2005-2010 Graduate Course, Keio University, Faculty of Science and Technology

DEGREES

B. Engineering (2005) Keio University
Dr. Engineering (2010) Keio University

APPOINTMENTS

2007 (Oct.) - 2010 (Mar.) Research Assistant, Global COE Program, Keio University
2010 (Apr.) - 2013 (Mar.) Postdoctoral Researcher, Chemical Biology Core Facility, RIKEN ASI
2013 (Apr.) - 2015 (Mar.)Special Postdoctoral Researcher, Antibiotics Laboratory, RIKEN
2015 (Apr.) – 2015 (Sep.)Special Postdoctoral Researcher, Chemical Biology Research Group, RIKEN CSRS
2015 (Oct.) – 2016 (Sep.)SContract Researcher, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, RIKEN Global Research Cluster
2016 (Oct.) – 2017 (Mar.)Postdoctoral Fellow, Department of Chemical Biology, Max-Planck-Institute of Molecular Physiology, Germany
[2015 (Sep.) – 2017 (Mar.)Worked in Department of Chemical Biology, Max-Planck-Institute of Molecular Physiology, Germany]
2017 (Apr.) –Research Scientist, RIKEN-Max Planck Joint Research Division for Systems Chemical Biology, RIKEN CSRS

ACADEMIC ACTIVITIES

  • Japan Society for Bioscience Biotechnology, and Agrochemistry (JSBBA)
  • The Japan Association for Molecular Target Therapy of Cancer (JAMTTC)
  • Japanese Cancer Association (JCA)

MY HOBBY

Playing Sports

LIST OF SELECTED PUBLICATIONS

  1. Julian Wilke, Tatsuro Kawamura, Nobumoto Watanabe, Hiroyuki Osada, Slava Ziegler, Herbert Waldmann. Identification of cytotoxic, glutathione-reactive moieties inducing accumulation of reactive oxygen species via glutathione depletion. Bioorg. Med. Chem. 26, 1453-1461 (2018)
  2. Ashutosh Kumar, Tatsuro Kawamura, Makoto Kawatani, Hiroyuki Osada, Kam Y. J. Zhang. Identification and structure-activity relationship of purine derivatives as novel MTH1 inhibitors. Chem. Biol. Drug Des. 89, 862-869 (2017)
  3. Tatsuro Kawamura, Makoto Kawatani, Makoto Muroi, Yasumitsu Kondoh, Yushi Futamura, Harumi Aono, Miho Tanaka, Kaori Honda, Hiroyuki Osada. Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival. Sci. Rep. 6, 26521 (2016)
  4. Tatsuro Kawamura, Yasumitsu Kondoh, Makoto Muroi, Makoto Kawatani, Hiroyuki Osada,
 A small molecule that induces reactive oxygen species via cellular glutathione depletion. Biochem. J. 463, 53-63 (2014)
  5. Yuichi Nakajima, Tatsuro Kawamura, Kazuyuki Maeda, Hinayo Ichikawa, Takayuki Motoyama, Yasumitsu Kohdoh, Tamio Saito, Tetsuo Kobayashi, Minoru Yoshida, Hiroyuki Osada, Makoto Kimura,
Identification and characterization of an inhibitor of trichothecene 3-O-acetyltransferase, TRI101, by the chemical array approach. Biosci. Biotechnol. Biochem. 77, 1958-1960 (2013)
  6. Tatsuro Kawamura, Kohei Matsubara, Hitomi Otaka, Etsu Tashiro, Kazutoshi Shindo, Ryo C. Yanagita, Kazuhiro Irie, Masaya Imoto, Generation of `Unnatural Natural Product' library and identification of a small molecule inhibitor of XIAP. Bioorg. Med. Chem. 19, 4377-4385 (2011)
  7. Tatsuro Kawamura, Takahiro Fujimaki, Natsuki Hamanaka, Kentaro Torii, Hiroki Kobayashi, Yoshikazu Takahashi, Masayuki Igarashi, Naoko Kinoshita, Yoshio Nishimura, Etsu Tashiro, Masaya Imoto, Isolation and structure elucidation of a novel androgen antagonist, arabilin, produced by Streptomyces sp. MK756-CF1. J. Antibiot. 63, 601-605 (2010)
  8. Tatsuro Kawamura, Etsu Tashiro, Kohta Yamamoto, Kazutoshi Shindo, Masaya Imoto, SAR study of a novel triene-ansamycin group compound, quinotrierixin, and related compounds, as inhibitors of ER stress-induced XBP1 activation, I. Taxonomy, fermentation, isolation, biological activities and SAR study. J. Antibiot. 61, 303-311 (2008)
  9. Tatsuro Kawamura, Etsu Tashiro, Kazutoshi Shindo, Masaya Imoto, SAR study of a novel triene-ansamycin group compound, quinotrierixin, and related compounds, as inhibitors of ER stress-induced XBP1 activation, II. Structure elucidation. J. Antibiot. 61, 312-317 (2008)

Yushi FUTAMURA


Yushi FUTAMURA
(Ph.D, Postdoctoral Researcher)

INTEREST OF MY RESEARCH

I have always imagined achieving the chemical biology study like Dr. Schreiber did using immuno-suppressive drug, FK506. What should I do for that? In general, chemical biology study starts by searching for unique bioactive compounds. I'm interested in cell morphology and also believe the microorganisms can produce many other compounds with chemical and functional diversities. I'm thus searching for fruitful bioactive compounds on the basis of their ability to alter cellular morphology from secondary metabolites of microorganisms.

EDUCATION

1998-2002 Keio University, Department of Applied Chemistry
2002-2008 Graduate Course, Keio University, Faculty of Science and Technology

DEGREES

B.Sc. (2002) Keio University
Dr. Science (2008) Keio University

APPOINTMENTS

2008-2008 Post Doctoral Fellow, Antibiotics Laboratory, RIKEN ASI
2008-2015 Special Post Doctoral Researcher, Antibiotics Laboratory, RIKEN ASI
2009-2013 Special Post Doctoral Researcher,Chemical Biology Core Facility, RIKEN ASI
2015.Apr.- RIKEN Center for Sustainable Resource Science - Chemical Biology Research Group

ACADEMIC ACTIVITIES

  • JSBBA
  • JCA
  • JAMTIC
  • Japanese Society for Chemical Biology

MY HOBBY

golf, lusty-singing

LIST OF SELECTED PUBLICATIONS

  1. Futamura Y, Muroi M, Aono H, Kawatani M, Hayashida M, Sekine T, Nogawa T, Osada H.
    Bioenergetic and proteomic profiling to screen small molecule inhibitors that target cancer metabolisms.
    Biochim. Biophys. Acta. (2018) in press
  2. Robke L, Futamura Y, Konstantinidis G, Wilke J, Aono H, Mahmoud Z, Watanabe N, Wu YW, Osada H, Laraia L, Waldmann H.
    Discovery of the novel autophagy inhibitor aumitin that targets mitochondrial complex I.
    Chem. Sci. (2018) 9: 3014-3022.
  3. Kaneko M, Futamura Y, Tsukuda S, Kondoh Y, Sekine T, Hirano H, Fukano K, Ohashi H, Saso W, Morishita R, Matsunaga S, Kawai F, Ryo A, Park SY, Suzuki R, Aizaki H, Ohtani N, Sureau C, Wakita T, Osada H, Watashi K.
    Chemical array system, a platform to identify novel hepatitis B virus entry inhibitors targeting sodium taurocholate cotransporting polypeptide.
    Sci. Rep. (2018) 8: 2769.
  4. Shikata Y, Kiga M, Futamura Y, Aono H, Inoue H, Kawada M, Osada H, Imoto M.
    Mitochondrial uncoupler exerts a synthetic lethal effect against β-catenin mutant tumor cells.
    Cancer Sci (2017) 108: 772-784.
  5. Futamura Y, Yamamoto K, Osada H.
    Phenotypic screening meets natural products in drug discovery.
    Biosci Biotechnol Biochem (2017) 81: 28-31.
  6. Minegishi H, Futamura Y, Fukashiro S, Muroi M, Kawatani M, Osada H, Nakamura H.
    Methyl 3-((6-methoxy-1,4-dihydroindeno[1,2-c]pyrazol-3-yl)amino) benzoate (GN39482) as a tubulin polymerization inhibitor identified by MorphoBase and ChemProteoBase profiling methods.
    J. Med. Chem. (2015) 58: 4230-4241.
  7. Futamura Y, Kawatani M, Muroi M, Nogawa T, Aono H, Osada H.
    Identification of a molecular target of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems.
    ChemBioChem (2013) 18: 2456-2463.
  8. Futamura Y, Kawatani M, Kazami S, Tanaka K, Muroi M, Shimizu T, Tomita K, Watanabe N, Osada H.
    Morphobase, an encyclopedic cell morphology database, and its use for drug target identification.
    Chem. Biol. (2012) 19: 1620-1630. COVER Image
  9. Sasazawa Y, Futamura Y, Tashiro E, Imoto M.
    V-ATPase inhibitors overcome Bcl-xL-mediated chemoresistance through restoration of a caspase-independent apoptotic pathway.
    Cancer Science (2009) 100: 1460-1467.
  10. Futamura Y, Sawa R, Umezawa Y, Igarashi M, Nakamura H, Hasegawa K, Yamasaki M, Tashiro E, Takahashi Y, Akamatsu Y, Imoto M.
    Discovery of Incednine as a Potent Modulator of the Anti-apoptotic Function of Bcl-xL from Microbial Origin.
    J. Am. Chem. Soc (2008) 130: 1822-1823.

Toshihiko NOGAWA


Toshihiko NOGAWA
(Ph.D, ASI Technical Scientist)

INTEREST OF MY RESEARCH

My research aim is to find a cure for the cancer from natural products, such as microorganisms, marine organisms, and plants.

EDUCATION

1990-1994Kanagawa University, Department of Biology
1998-2001 Graduate Course, Kanagawa University, Faculty of Biology

DEGREES

B.Sc. (1994) Kanagawa University 
Dr. Sc. (2001) Kanagawa University 

APPOINTMENTS   

1996-1998Researcher, Kobayashi Pharmaceutical Industrial Co. Ltd.
2001-2001 Research Fellow, Research Institute for Integrated Science, Kanagawa University.
2001-2006Postdoc & Research associate, Cancer Research Institute, Arizona State University.
2006-2013Postdoc, Antibiotics Laboratory, RIKEN.
2009-2013Postdoc,Chemical Biology Core Facility, RIKEN ASI  
2013-Research Scientist, RIKEN Center for Sustainable Resource Science - Chemical Biology Research Group

MY HOBBY

Treasure hunt

LIST OF SELECTED PUBLICATION

  1. Kamano Y., Nogawa T., Kotake A,. Tozawa M., Pettit G. R.
    Separation of toad poison bufadienolides by hydrophobic gel.
    J. Liq. Chrom. & Rel. Technol., 22 (16), 2455-2456 (1999)

  2. Nogawa T., Kamano Y., Yamashita A., Pettit G. R.
    Isolation and structure of five new cancer cell growth inhibitory bufadienolides from the Chinese traditional drug Ch'an Su.
    J. Nat. Prod., 64, 1148-1152 (2001)

  3. Kamano Y., Nogawa T., Yamashita A., Hayashi M., Inoue M., Drasar P., Pettit G. R.
    Isolation and structure of a 20,21-epoxybufaenolide series from "Ch'an Su".
    J. Nat. Prod., 65, 1001-1005 (2002)

  4. Pettit G. R., Nogawa T., Knight J. C., Doubek D., Hooper J. N. A.
    Antineoplastic agents. 535. Isolation and Structure of plakorstatins 1 and 2 from the Indo-Pacific sponge Plakortis nigra.
    J. Nat. Prod., 67, 1611-1613 (2004)

  5. LaMarca H. L., Morris C. A., Pettit G. R., Nogawa T., Puschett J. B.
    Marinobufagenin impairs first trimester cytotrophoblast differentiation.
    Placenta, 27 (9-10), 984-988 (2006)

Takeshi SHIMIZU

  
Takeshi SHIMIZU
(Ph.D, Special Temporary Employee)

INTEREST OF MY RESEARCH

Microorganisms produce various compounds with chemical and biological diversities. I want to chemically synthesize useful compounds which microorganisms can not produce.

EDUCATION

1967-1971Tohoku University, Department of Pharmaceutical Sciences
1971-1973Tohoku University, Faculty of Pharmaceutical Sciences

DEGREES

B.Sc. (1971) Tohoku University 
Dr. Pharmacy (1979) Tohoku University 

APPOINTMENTS

1973-Reserch Scientist, Synthetic Pesticide Laboratory I, RIKEN
1981-1983Research Fellow, Columbia University, USA.
1992- Senior Reserch Scientist, RIKEN
1998-2001 Visiting Professor, Rikkyo University
2001- present Visiting Professor, Tokyo Denki University
2001-2009 Senior Scientist, Synthetic Organic Chemistry, RIKEN
2004-2008Team Leader, RIKEN Chemical Biology Group
2009-2009Special Temporary Employee, RIKEN Chemical Biology Department
2009-2013Special Temporary Employee,Chemical Biology Core Facility, RIKEN ASI  
2013-2015Special Temporary Employee, Antibiotics Lab.
2015.Apr.-RIKEN Center for Sustainable Resource Science - Chemical Biology Research Group

ACADEMIC ACTIVITIES

  • The Pharmaceutical Society of Japan
  • The Society of Synthetic Organic Chemistry, Japan
  • Japanese Society for Chemical Biology<

MY HOBBY

Gardening

LIST OF SELECTED PUBLICATIONS

  1. Nogawa, T., Kato,, N., Shimizu, T., Okano, A., Futamura, Y., Takahashi, S., Osada, H.,
    "Wakodecalines A and B, new decaline metabolites isolated from a fungus Pyrenochaetopsis sp. RK10-F058"
    J. Antibiot. 2018, 71, 123-128.

  2. Kondoh, Y.; Honda, K.; Hiranuma, H.; Hayashi, T.; Shimizu, T.; Watanabe, N.; Osada, H.
    "Comparative chemical array screening for p38γ/δ MAPK inhibitors using a single gatekeeper residue difference between p38α/β and p38γ/δ" Sci. Rep. 2016, 6, 29881.

  3. Subedi, A.; Shimizu, T.; Ryo, A.; Sanada, E.; Osada, H., Watanabe, N. “Discovery of novel selenium derivatives as Pin1 inhibitors by high-throughput screening” Biochem. Biophysic. Res. Commun. 2016, 474, 528-533.

  4. Yao, R.; Kondoh, Y.; Natsume, Y.; Yamanaka, H.; Inoue, M.; Toki, H.; Takagi, R.; Shimizu, T.; Yamori, T.; Osada, H.; Noda, T. " A small compound targeting TACC3 revealed its different spatiotemporal contributions for spindle assembly in cancer cells" Oncogene. 2013, 14, 4242-4252.

  5. Zimmermann, T. J.; Bürger, M.; Tashiro, E.; Kondoh, Y.; Martinez, N. E.; Görmer, K.; Rosin-Steiner, S.; Shimizu, T.; Ozaki, S.; Mikoshiba, K.; Watanabe, N.; Hall, D.; Vetter, I. R.; Osada, H.; Hedberg, C., Waldmann, H. " Boron-Based Inhibitors of Acyl Protein Thioesterase 1 and 2" ChemBioChem. 2013, 14, 115-122.

  6. Kahn, M. M. G.; Simizu, S; Lai, N. S.; Kawatani, M.; Shimizu, T.; Osada, H. " Discovery of a Small Molecule PDI Inhibitor that Inhibits Reduction of HIV-1 Envelope Gycoprotein gp120 ", Chem. Biol. 2011, 6, 245-251.

  7. Shimizu, T.; Usui, T.; Fujikura, M.; Kawatani, M.; Satoh, T.; Machida, K.; Kanoh, N.; Woo, J-T.; Osada, H.; Sodeoka, M. "Synthesis and Biological Activities of Reveromycin A and Spirofungin A Derivatives", Bioorg. Med. Chem. Lett. 2008, 18, 3756-3760.

  8. Shimizu, T.; Satoh, T.; Murakoshi, K.; Sodeoka, M. " Asymmetric Total Synthesis of (-)-Spirofungin A and (+)-Spirofungin B ", Org. Lett. 2005, 7, 4965-4968.

  9. Shimizu, T.; Masuda, T.; Hiramoto, K.; Nakata, T. "Total Synthesis of Reveromysin A ", Org. Lett. 2000, 2, 2153-2156.

  10. Shimizu, T.; Ohzeki, T.; Hiramoto, K.; Hori, N.; Nakata, T. " Chloromethanesulfonate as an Efficient Leaving Group: Rearrangement of the Carbon-Carbon Bond and Conversion of Alcohols into Azides and Nitrils ", Synthesis 1999, 1373-1385.

Takayuki MOTOYAMA

Takayuki Motoyama
Takayuki MOTOYAMA
(Ph.D,Senior Research Scientist)

INTEREST OF MY RESEARCH

Filamentous fungi (molds) are well known as plant and animal pathogens and mycotoxin producers. On the other hand, filamentous fungi are useful in production of fermentation foods (sake etc.) and medicines. Recently, it has become apparent that huge numbers of secondary metabolism genes of unknown functions are sleeping in the genomes of filamentous fungi. Aims of my research are to develop medicines by using these genes and to develop agrochemicals that control production of fungal secondary metabolites required for pathogenicity.

EDUCATION

1986-1990  The University of Tokyo, Department of Agricultural Chemistry
1990-1996 Graduate Course, The University of Tokyo, Faculty of Agriculture

DEGREES

B.Sc. (1990)  The University of Tokyo 
Dr. Agriculture (1995)  The University of Tokyo 

APPOINTMENTS

1995-2002 Scientist, Microbial Toxicology Lab., RIKEN
2002-2008 Senior Scientist, Environmental Molecular Biology Lab., RIKEN
2008-2015 Senior Scientist, Antibiotics Lab., RIKEN
2014.Feb.-RIKEN Center for Sustainable Resource Science - Chemical Biology Research Group

ACADEMIC ACTIVITIES

  • Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Fungal Molecular Biology Society of Japan
  • Japanese Society for Chemical Biology

MY HOBBY

Appreciation and using of ceramic arts, cat

LIST OF SELECTED PUBLICATIONS

  1. Yun, C.-S, Motoyama, T. amd Osada, H.: "Regulatory mechanism of mycotoxin tenuazonic acid production in Pyricularia oryzae", ACS Chem Biol, 12: 2270-2274 (2017).
    Press release: http://www.riken.jp/pr/press/2017/20171006_1/
  2. Yun, C.-S., Motoyama, T. and Osada, H.: "Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme", Nat Commun, 6: 8758 (2015).
    Press release: http://www.riken.jp/pr/press/2015/20151027_1/
  3. Motoyama, T., Hayashi, T., Hirota, H., Ueki, M. and Osada, H.:" Terpendole E, a Kinesin Eg5 Inhibitor, Is a Key Biosynthetic Intermediate of Indole-Diterpenes in the Producing Fungus Chaunopycnis alba", Chemistry & Biology, 19: 1611-1619, (2012).
    Press release: http://www.riken.jp/pr/press/2012/20121221_2/
  4. Vetcher,, L., Hugo G. Menzella, H.G., Kudo, T., Motoyama, T., and Katz, L.: “
The Antifungal Polyketide Ambruticin Targets the HOG Pathway”, 
Antimicrob. Agents Chemother., 51: 3734-3736 (2007).
  5. Motoyama, T., Ohira, T., Kadokura, K., Ichiishi, A., Fujimura, M., Yamaguchi, I., Kudo, T.: “An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast
”, Curr. Genet., 47: 298-306 (2005).

Makoto MUROI


Makoto MUROI
(Ph.D,Senior Research Scientist)

INTEREST OF MY RESEARCH

Many kind of biological active compounds are isolated from microorganisms, and compounds with new targets provide new insights into biology. I'm interested in the target analysis of such compounds by proteomics.

EDUCATION

1984-1988 The University of Tokyo, Department of Agricultural Chemistry 
1988-1993 Graduate Course, The University of Tokyo, Faculty of Agriculture 

DEGREES

B.Sc. (1988) The University of Tokyo 
Dr. Agriculture (1993) The University of Tokyo 

APPOINTMENTS

1993-2003 Scientist, Animal and Cellular Systems Laboratory, RIKEN 
2003-2015Senior Scientist, Antibiotics Laboratory, RIKEN 
2009-2013Senior Scientist, Chemical Biology Core Facility, RIKEN ASI
2013.Apr.-RIKEN Center for Sustainable Resource Science - Chemical Biology Research Group

ACADEMIC ACTIVITIES

Japan Society for Bioscience, Biotechnology, and Agrochemistry
Japanese Cancer Association
The Japanese Association for Molecular Target Therapy of Cancer

LIST OF SELECTED PUBLICATIONS

  1. Futamura Y, Muroi M, Aono H, Kawatani M, Hayashida M, Sekine T, Nogawa T, Osada H.
    Bioenergetic and proteomic profiling to screen small molecule inhibitors that target cancer metabolisms.
    Biochim Biophys Acta, (doi: 10.1016/j.bbapap.2018.06.001)
  2. Khalid A, Takagi H, Panthee S, Muroi M, Chappell J, Osada H, Takahashi S.
    Development of a Terpenoid-Production Platform in Streptomyces reveromyceticus SN-593.
    ACS Synth Biol 6, 2339-2349 (2017)
  3. Robke L, Laraia L, Carnero Corrales MA, Konstantinidis G, Muroi M, Richters A, Winzker M, Engbring T, Tomassi S, Watanabe N, Osada H, Rauh D, Waldmann H, Wu YW, Engel J.
    Phenotypic Identification of a Novel Autophagy Inhibitor Chemotype Targeting Lipid Kinase VPS34.
    Angew Chem Int Ed Engl 56, 8153-8157 (2017)
  4. Kawatani M, Muroi M, Wada A, Inoue G, Futamura Y, Aono H, Shimizu K, Shimizu T, Igarashi Y, Takahashi-Ando N, Osada H.
    Proteomic profiling reveals that collismycin A is an iron chelator.
    Sci Rep 6, 38385 (2016)
  5. Muroi M, Futamura Y, Osada H.
    Integrated profiling methods for identifying the targets of bioactive compounds: MorphoBase and ChemProteoBase.
    Nat Prod Rep 33, 621-5 (2016)
  6. Kawamura T, Kondoh Y, Muroi M, Kawatani M, Osada H.
    A small molecule that induces reactive oxygen species via cellular glutathione depletion.
    Biochem J 463, 53-63 (2014)
  7. Futamura Y, Kawatani M, Muroi M, Aono H, Nogawa T, Osada H.
    Identification of a molecular target of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems.
    Chembiochem 14, 2456-63 (2013)
  8. Futamura Y, Muroi M, Osada H.
    Target identification of small molecules based on chemical biology approaches.
    Molecular bioSystems 9, 897-914 (2013)
  9. Osada H, Muroi M, Yoshida M.
    The chemistry/biology interface.
    Bioorg Med Chem 20, 1844-1845 (2012)
  10. Sasazawa Y, Kanagaki S, Tashiro E, Nogawa T, Muroi M, Kondoh Y, Osada H, Imoto M.
    Xanthohumol impairs autophagosome maturation through direct inhibition of valosin-containing protein.
    ACS Chem Biol 7, 892-900 (2012)
  11. Futamura Y, Kawatani M, Kazami S, Tanaka K, Muroi M, Shimizu T, Tomita K, Watanabe N, Osada H.
    Morphobase, an Encyclopedic Cell Morphology Database, and Its Use for Drug Target Identification.
    Chem Biol 19, 1620-1630 (2012).
  12. Asami Y, Jang JH, Soung NK, He L, Moon DO, Kim JW, Oh H, Muroi M, Osada H, Kim BY, Ahn JS.
    Protuboxepin A, a marine fungal metabolite, inducing metaphase arrest and chromosomal misalignment in tumor cells.
    Bioorg Med Chem 20, 3799-3806 (2012)
  13. Jang JH, Asami Y, Jang JP, Kim SO, Moon DO, Shin KS, Hashizume D, Muroi M, Saito T, Oh H, Kim BY, Osada H, Ahn JS.
    Fusarisetin A, an acinar morphogenesis inhibitor from a soil fungus, Fusarium sp. FN080326.
    J Am Chem Soc 133, 6865-6867 (2011)
  14. Kawatani M, Takayama H, Muroi M, Kimura S, Maekawa T, Osada H.
    Identification of a small-molecule inhibitor of DNA topoisomerase II by proteomic profiling.
    Chem Biol 18, 743-751 (2011).
  15. Muroi M, Kazami S, Noda K, Kondo H, Takayama H, Kawatani M, Usui T, Osada H.
    Application of proteomic profiling based on 2D-DIGE for classification of compounds according to the mechanism of action.
    Chem Biol 17, 460-470 (2010)

Makoto KAWATANI


Makotoo KAWATANI
(Ph.D, Senior Research Scientist)
INTEREST OF MY RESEARCH
Microorganisms often produce useful compounds for us. I have screened such compounds, especially anticancer compounds, and have studied their mode of action. I want to contribute to development of new drugs through chemical biology research.
EDUCATION
1994-1998
Faculty of Science and Technology, Keio University
2001-2003
JSPS research fellowships for young scientists
1998-2003
Graduate School of Science and Technology, Keio University
DEGREES
B.Sc. (1998)
Keio University
Dr. Science (2003)
Keio University
APPOINTMENTS
2003-2004
Postdoctoral Researcher, Antibiotics Laboratory, RIKEN
2004-2007
Special Postdoctoral Researcher, Antibiotics Laboratory, RIKEN
2007-2009
Postdoctoral Researcher, Antibiotics Laboratory, RIKEN
2009-2012
Research Scientist, Antibiotics Laboratory, RIKEN
2012-2015
Senior Research Scientist, Chemical Biology Core Facility, RIKEN ASI
2015.Apr.-
Senior Research Scientist, Chemical Biology Research Group, RIKEN CSRS
AWARDS
  • The Excellent Paper Award Published in Bioscience, Biotechnology, & Biochemistry (2007)
  • Best Presentation Award, 2007 Annual Meeting of the Japanese Association for Molecular Target Therapy of Cancer (2007)
  • Research Incentive Award of RIKEN (2009)
  • The Excellent Paper Award Published in Bioscience, Biotechnology, & Biochemistry (2009)
  • The Research Incentive Award of the Japanese Association for Molecular Target Therapy of Cancer (2009)
  • Japanese Cancer Association Incitement Award (2012)
ACADEMIC ACTIVITIES
  • Japanese Cancer Association
  • The Japanese Association for Molecular Target Therapy of Cancer (Councilor)
  • Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Japanese Society for Chemical Biology
  • Oncology Research (Editorial Board Member)
MY HOBBY
Football, Badminton
LIST OF SELECTED PUBLICATIONS
  1. Kawamura T.*, Kawatani M.*, Muroi M., Kondoh Y., Futamura Y., Aono H., Tanaka M., Honda K., and Osada H. Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival. Sci. Rep. 6, 26521 (2016) *, equal contribution
  2. Kawatani M., Fukushima Y., Kondoh Y., Honda K., Sekine T., Yamaguchi Y., Taniguchi N., and Osada H. Identification of matrix metalloproteinase inhibitors by chemical arrays. Biosci. Biotechnol. Biochem. 79, 1597-1602 (2015)
  3. Kawatani M., and Osada H. Affinity-based target identification for bioactive small molecules. MedChemComm 5, 277-287 (2014) Review Article
  4. Futamura Y., Kawatani M., Muroi M., Aono H., Nogawa T., and Osada H. Molecular target identification of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems. Chembiochem 14, 2456-2463 (2013)
  5. Futamura Y.*, Kawatani M.*, Kazami S., Tanaka K., Muroi M., Shimizu T., Tomita K., Watanabe N., and Osada H. Morphobase, an encyclopedic cell morphology database, and its use for drug target identification. Chem. Biol. 19, 1620-1630 (2012) *, equal contribution
  6. Kawatani M., Takayama H., Muroi M., Kimura S., Maekawa T., and Osada H. Identification of a small-molecule inhibitor of DNA topoisomerase II by proteomic profiling. Chem. Biol. 18, 743-751 (2011)
  7. Kawatani M., and Osada H. Osteoclast-targeting small molecules for the treatment of neoplastic bone metastases. Cancer Sci. 100, 1999-2005 (2009) Review Article
  8. Kawatani M., Okumura H., Honda K., Kanoh N., Muroi M., Dohmae N., Takami M., Kitagawa M., Futamura Y., Imoto M., and Osada H. The identification of an osteoclastogenesis inhibitor through the inhibition of glyoxalase I. Proc. Natl. Acad. Sci. USA 105, 11691-11696 (2008)
  9. Woo J.-T.*, Kawatani M.*, Kato M., Shinki T., Yonezawa T., Kanoh N., Nakagawa H., Takami M., Lee K. H., Stern P. H., Nagai K., and Osada H. Reveromycin A, an agent for osteoporosis, inhibits bone resorption by inducing apoptosis specifically in osteoclasts. Proc. Natl. Acad. Sci. USA 103, 4729-4734 (2006) *, equal contribution
  10. Kawatani M., and Imoto M. Deletion of the BH1 domain of Bcl-2 accelerates apoptosis by acting in a dominant negative fashion. J. Biol. Chem. 278, 19732-19742 (2003)

Naoki KATO

Naoki KATO
Naoki KATO
(Ph.D, Research Scientist)

Chemical Resource Development Research Unit

INTEREST OF MY RESEARCH

Filamentous fungi produce secondary metabolites with complex structures and various biological activities. Aims of my research are to elucidate the biosynthetic mechanisms of bioactive fungal secondary metabolites and expand their chemical diversity by pathway engineering. To accelerate the utilization of microbial genetic resources, I am also working on construction of a biosynthetic platform for the production of valuable chemicals and chemical activation of the fungal secondary metabolite production.

EDUCATION
1993-1997
Faculty of Agriculture, Hokkaido University
1997-1999
Graduate School of Agriculture, Hokkaido University
1999-2002
Graduate School of Bioagricultural Sciences, Nagoya University
DEGREES
Ph.D. (2002)
Graduate School of Bioagricultural Sciences, Nagoya University
APPOINTMENTS
2002-2003
Research associate, Department of Biological Sciences, Northern Illinois University
2004-2005
NEDO Research fellow, Forestry Research Institute, Oji Paper Company Ltd.
2005-2007
Postdoctoral Researcher, Antibiotics Laboratory, RIKEN
2007-2010
Special Postdoctoral Researcher, Antibiotics Laboratory, RIKEN
2010-2013
ASI Research Scientist, Chemical Biology Core Facility, RIKEN ASI
2010-2011
Visiting Scientist, Dept. of Chemical Biology, Max-Planck-Institute of Molecular Physiology
2013.Apr.-
Research Scientist, Natural Product Biosynthesis Research Unit, Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science
ACADEMIC ACTIVITIES
  • Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Fungal Molecular Biology Society of Japan
LIST OF SELECTED PUBLICATIONS
  1. Kato N., Nogawa T., Hirota H., Jang JH, Takahashi S., Ahn JS, Osada H.: A new enzyme involved in the control of the stereochemistry in the decalin formation during equisetin biosynthesis. Biochem Biophys Res Commun, 460: 210-215 (2015)
  2. Kato N., Takahashi S., Nogawa T., Saito T., Osada H., Construction of a microbial natural product library for chemical biology studies. Curr. Opin. Chem. Biol.,16, 101-108 (2012) (review)
  3. Kato N., Tokuoka M., Shinohara Y., Kawatani M., Uramoto M., Seshime Y., Fujii I., Kitamoto K., Takahashi T., Koyama Y., and Osada H., Genetic safeguard against mycotoxin cyclopiazonic acid production in Aspergillus oryzae. ChemBioChem, 12, 1376-1382 (2011).
  4. Kato N., Suzuki H., Takagi H., Uramoto M., Takahashi S., and Osada H., Gene disruption and biochemical characterization of verruculogen synthase of Aspergillus fumigatus. ChemBioChem, 12, 711-714 (2011).
  5. Kato N., Suzuki, H., Takagi, H., Asami, Y., Kakeya, H., Uramoto, M., Usui, T., Takahashi, S., Sugimoto, Y., and Osada, H., Identification of cytochrome P450s required for fumitremorgin biosynthesis in Aspergillus fumigatus. Chembiochem 10, 920-928 (2009).
  6. Kato N., Brooks, W., and Calvo, A. M., The expression of sterigmatocystin and penicillin genes in Aspergillus nidulans is controlled by veA, a gene required for sexual development. Eukaryot. Cell 2, 1178-1186 (2003).

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