RIKEN Center for Sustainable Resource Science
Chemical Biology Group (Archives)

Hiroyuki OSADA - Group Director

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Hiroyuki OSADA

RIKEN Center for Sustainable Resource Science
Group Director of Chemical Biology Research Group

INTEREST OF MY RESEARCH

I wonder why microorganisms produce various compounds with chemical and biological diversities. To resolve the mystery, I am interested in biosynthesis gene clusters of microorganisms. In addition, I want to contribute to the society through the chemical biology research with bioprobes and the molecular target therapy of tumors.

EDUCATION

1974-1978

The University of Tokyo, Department of Agricultural Chemistry

1978-1983

Graduate Course, The University of Tokyo, Faculty of Agricul
Dr. Agriculture (1983) The University of Tokyo

APPOINTMENTS

1983-1991

Scientist, Antibiotics Laboratory, RIKEN

1985-1986

Research Fellow, National Cancer Institute, NIH, USA.

1992-2015

Chief Scientist, Antibiotics Laboratory, RIKEN

1999- 2020

Visiting Professor, Saitama University

2009-2013

Director, Chemical Biology Core Facility, RIKEN ASI

2013- 2020

Deputy Director, RIKEN Center for Sustainable Resource Science

2013- present

Director, Chemical Biology Research Group,

ACADEMIC ACTIVITIES

Editorial Board Member

• Assay and Drug Development Technology
• Cancer Science
• Journal of Antibiotics
• Journal of General and Applied Microbiology
• Journal of Industrial Microbiology & Biotechnology
• Journal of Microbiology and Biotechnology
• Oncology Research

Advisory Board Member

• ACS Chemical Biology

MY HOBBY

Chatting with drinking wine

AWARDS

• Research Promotion Award of Agricultural Chemical Society of Japan (1991)
• Sumiki-Umezawa Memorial Award from Japan Antibiotic Research Association (1996)
• Award of the Society for Actinomycetes Japan (2000)
• Award of the Minister of Education, Culture, Sports, Science and Technology (2002)
• Award of the Bioindustry Association (2007)
• Award of Agricultural Chemical Society of Japan (2009)
• Significant Achievement Award (S) RIKEN (2010)
• Inhoffen Medal (2015)
• Special Award of Agricultural Chemical Society of Japan (2016)

SELECTED PAPERS

1. Nagasawa I, Muroi M, Kawatani M, Ohishi T, Ohba SI, Kawada M & Osada H.: Identification of a small compound targeting PKM2-regulated signaling using 2D gel electrophoresis-based proteome-wide CETSA. Cell Chem Biol, 27(2), 186-196.e4 (2020) [ doi: 10.1016/j.chembiol.2019.11.010 ]
2. Simpkins SW, Deshpande R, Nelson J, Li SC, Piotrowski JS, Ward HN, Yashiroda Y, Osada H, Yoshida M, Boone C & Myers CL.: Using BEAN-counter to quantify genetic interactions from multiplexed barcode sequencing experiments. Nat Protoc, 14(2): 415-440 (2019) [ doi: 10.1038/s41596-018-0099-1 ]
3. Piotrowski JS, Li SC, Deshpande R, Simpkins SW, Nelson J, Yashiroda Y, Barber JM, Safizadeh H, Wilson E, Okada H, Gebre AA, Kubo K, Torres NP, LeBlanc MA, Andrusiak K, Okamoto R, Yoshimura M, DeRango-Adem E, van Leeuwen J, Shirahige K, Baryshnikova A, Brown GW, Hirano H, Costanzo M, Andrews B, Ohya Y, Osada H, Yoshida M, Myers CL & Boone C.: Functional annotation of chemical libraries across diverse biological processes. Nat Chem Biol, 13(9): 982-993 (2017) [ doi: 10.1038/nchembio.2436 ]
4. Ursu A, Illich DJ, Takemoto Y, Porfetye AT, Zhang M, Brockmeyer A, Janning P, Watanabe N, Osada H, Vetter IR, Ziegler S, Schöler HR & Waldmann H.: Epiblastin A induces reprogramming of epiblast stem cells into embryonic stem cells by inhibition of casein kinase 1. Cell Chem Biol, 23(4): 494-507 (2016) [ doi: 10.1016/j.chembiol.2016.02.015 ]
5. Ray L, Valentic TR, Miyazawa T, Withall DM, Song L, Milligan JC, Osada H, Takahashi S, Tsai SC & Challis GL.: A crotonyl-CoA reductase-carboxylase independent pathway for assembly of unusual alkylmalonyl-CoA polyketide synthase extender units. Nat Commun, 7: 13609 (2016) [ doi: 10.1038/ncomms13609 ]
6. Hasebe F, Matsuda K, Shiraishi T, Futamura Y, Nakano T, Tomita T, Ishigami K, Taka H, Mineki R, Fujimura T, Osada H, Kuzuyama T & Nishiyama T.: Amino group carrier protein-mediated secondary metabolite biosynthesis in Streptomyces Nat Chem Biol, 12: 967–972 (2016) [ doi: 10.1038/nchembio.2181 ]
7. Yun CS, Motoyama T & Osada H.: Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme. Nat Commun, 6: 8758 (2015) [ doi: 10.1038/ncomms9758 ]
8. Soeda Y, Yoshikawa M, Almeida OF, Sumioka A, Maeda S, Osada H, Kondoh Y, Saito A, Miyasaka T, Kimura T, Suzuki M, Koyama H, Yoshiike Y, Sugimoto H, Ihara Y & Takashima A.: Toxic tau oligomer formation blocked by capping of cysteine residues with 1,2-dihydroxybenzene groups. Nat Commun, 6: 10216 (2015) [ doi: 10.1038/ncomms10216 ]
9. Takahashi S, Toyoda A, Sekiyama Y, Takagi H, Nogawa T, Uramoto M, Suzuki R, Koshino H, Kumano T, Panthee S, Dairi T, Ishikawa J, Ikeda H, Sakaki Y & Osada H.: Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. Nat Chem Biol. 7: 461-468 (2011) [ doi: 10.1038/nchembio.583 ]
10. Sun Y, Hahn F, Demydchuk Y, Chettle J, Tosin M, Osada H & Leadlay PF : In vitro reconstruction of tetronate RK-682 biosynthesis. Nature Chem Biol, 6, 99-101 (2010) [ doi: 10.1038/nchembio.285 ]
11. Miyazaki I, Simizu S, Okumura H, Takagi S & Osada H: A small-molecule inhibitor shows that pirin regulates migration of melanoma cells. Nature Chem Biol., 6,667-673,(2010) [ doi: 10.1038/nchembio.423 ]
12. Yano A, Tsutsumi S, Soga S, Lee MJ, Trepel J, Osada H & Neckers L.: Inhibition of Hsp90 activates osteoclast c-Src signaling and promotes growth of prostate carcinoma cells in bone. Proc Natl Acad Sci U S A, 105(40): 15541-15546 (2008) [ doi: 10.1073/pnas.0805354105 ]
13. Kawatani M, Okumura H, Honda K, Kanoh N, Muroi M, Dohmae N, Takami M, Kitagawa M, Futamura Y, Imoto M & Osada H.: The identification of an osteoclastogenesis inhibitor through the inhibition of glyoxalase I. Proc Natl Acad Sci U S A, 105(33): 11691-11696 (2008) [ doi: 10.1073/pnas.0712239105 ]
14. Woo JT, Kawatani M, Kato M, Shinki T, Yonezawa T, Kanoh N, Nakagawa H, Takami M, Lee KH, Stern PH, Nagai K & Osada H.: Reveromycin A, an agent for osteoporosis, inhibits bone resorption by inducing apoptosis specifically in osteoclasts. Proc Natl Acad Sci U S A, 103(12): 4729-4734 (2006) [ doi: 10.1073/pnas.0505663103 ]
15. Watanabe N, Arai H, Nishihara Y, Taniguchi M, Watanabe N, Hunter T & Osada H.: M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP. Proc Natl Acad Sci U S A, 101(13): 4419-4424 (2004) [ doi: 10.1073/pnas.0307700101 ]
16. Watanabe N, Arai H, Iwasaki J, Shiina M, Ogata K, Hunter T & Osada H.: Cyclin-dependent kinase (CDK) phosphorylation destabilizes somatic Wee1 via multiple pathways. Proc Natl Acad Sci U S A, 102(33): 11663-11668 (2005) [ doi: 10.1073/pnas.0500410102 ]
17. Simizu S & Osada H.: Mutations in the Plk gene lead to instability of Plk protein in human tumour cell lines. Nat Cell Biol, 2(11): 852-854 (2000) [ doi: 10.1038/35041102 ]

SELECTED REVIEWS

1. Muroi M & Osada H: Proteomic profiling for target identification of biologically active small molecules using 2D DIGE. Methods Mol Biol, 1888, 127-139 (2019) [ doi: 10.1007/978-1-4939-8891-4_7 ]
2. Osada H.: Trends in Bioprobe Research.Bioprobes (Ed. H. Osada), Springer, 1-10 (2017) [ doi: 10.1007/978-4-431-56529-1_1 ]
3. Watanabe N & Osada H.: Small molecules that target phosphorylation dependent protein-protein interaction. Bioorg Med Chem, 24(15): 3246-3254 (2016) [ doi: 10.1016/j.bmc.2016.03.023 ]
4. Osada H.: Chemical and biological studies of reveromycin A. J Antibiot, 69(10): 723-730 (2016) [ doi: 10.1038/ja.2016.57 ]
5. Muroi M, Futamura Y, & Osada H.: Integrated profiling methods for identifying the targets of bioactive compounds: MorphoBase and ChemProteoBase. Nat Prod Rep, 33(5): 621-625 (2016) [ doi: 10.1039/c5np00106d ]