Pyrrolizilactone
- Structure
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- Producing organism
- an uncharacterized fungus
- Biological activity
- proteasome inhibitor
- Abstract
- In the course of screening our microbial metabolite fraction library, we identified a novel pyrrolizidinone compound, pyrrolizilactone. In this study, we report the identification and characterization of a molecular target for pyrrolizilactone by using two phenotypic profiling systems. Cell morphology-based profiling analysis using an imaging cytometer (MorphoBase) classified pyrrolizilactone as a proteasome inhibitor. Consistently, proteome-based profiling analysis using 2D difference gel electrophoresis (DIGE; ChemProteoBase) also demonstrated that pyrrolizilactone is associated with proteasome inhibition. On the basis of these predictions, we determined that pyrrolizilactone is a novel type of proteasome inhibitor inhibiting the trypsin-like activity of the proteasome.
- References
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- Nogawa T, Kawatani M, Uramoto M, Okano A, Aono H, Futamura Y, Koshino H, Takahashi S, Osada H.: Pyrrolizilactone, a new pyrrolizidinone metabolite produced by a fungus.
J Antibiot, 66(10): 621-623 (2013)
[ doi: 10.1038/ja.2013.55 ]- Futamura Y, Kawatani M, Muroi M, Aono H, Nogawa T, Osada H.: Identification of a molecular target of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems.
Chembiochem, 14(18): 2456-2463 (2013) [ doi: 10.1002/cbic.201300499 ] - Nogawa T, Kawatani M, Uramoto M, Okano A, Aono H, Futamura Y, Koshino H, Takahashi S, Osada H.: Pyrrolizilactone, a new pyrrolizidinone metabolite produced by a fungus.