Guanine-7-N-oxide
- Structure
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- Producing organism
- Streptomyces sp.
- Biological activity
- cytotoxic activity
- Abstract
- Guanine 7-N-oxide produced by Streptomyces sp. was found to inhibit in vitro the replication of herpes virus (Oncorhynchus masou virus, OMV), rhabdo virus (infectious hematopoietic necrosis virus, IHNV) and a bi-segmented double-strand virus (infectious pancreatic necrosis virus, IPNV) derived from salmonids with IC50 values of about 10 µg/ml, 20 µg/ml and 32 µg/ml, respectively. The agent was not toxic for the host cells (chinook salmon embryo, CHSE-214) at the IC50 concentrations. Labeling of IHNV viral RNA and host cellular DNA and RNA with [3H]uridine and [3H]thymidine during drug treatment showed that guanine 7-N-oxide did not reduce the incorporation of these precusors into RNA and DNA. The anti-IHNV activity of guanine 7-N-oxide was enhanced synergistically by neplanocin A, an inhibitor of RNA methylation. The mechanism of action of guanine 7-N-oxide is discussed, in regard to maturation of viral messenger RNA including capping.
- References
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isolation and structural determination
- Nishii M, Inagaki J, Nohara F, Isono K, Kusakabe H, Kobayashi K, Sakurai T, Koshimura S, Sethi SK, McCloskey JA.: A new antitumor antibiotic, guanine 7-N-oxide produced by Streptomyces sp.
J Antibiot, 38(10): 1440-1443 (1985)
biological activities- Hasobe M, Saneyoshi M, Isono K.: Antiviral activity and its mechanism of guanine 7-N-oxide on DNA and RNA viruses derived from salmonid.
J Antibiot, 38(11): 1581-1587 (1985)- Hasobe M, Saneyoshi M, Isono K.: The synergism of nucleoside antibiotics combined with guanine 7-N-oxide against a rhabdovirus, infectious hematopoietic necrosis virus (IHNV).
J Antibiot, 39(9): 1291-1297 (1986) - Nishii M, Inagaki J, Nohara F, Isono K, Kusakabe H, Kobayashi K, Sakurai T, Koshimura S, Sethi SK, McCloskey JA.: A new antitumor antibiotic, guanine 7-N-oxide produced by Streptomyces sp.