Small molecule inhibitors of cell cycle regulatory proteins

Nobumoto Watanabe
Unit Leader
Nobumoto Watanabe
We also study an HIV-1 encoded protein, Vpr, that also inhibits the entry of mitosis. Vpr is important in AIDS pathogenesis, therefore we would like to know its mode of action. Using the knowledge about the action of these cell cycle regulatory proteins, we are currently focusing on the isolation of small molecule inhibitors of them. We recently established a screening system for the inhibitors of phosphorylation dependent protein-protein interaction such as polo box domain (PBD) dependent binding that is important for the Wee1 degradation upon the onset of mitosis. We also made a screening system for Vpr inhibitors using budding yeast, Saccharomyces cerevisiae, and isolated inhibitors of Vpr.