Dephospho-phoslactomycin B

Producing organism
Streptomyces sp.
Biological activity
differentiation inducer, enhancer of differentiation potential of all-trans retinoic acid.
During the screening for novel differentiation inducers, we found that a culture broth of Streptomyces sp. HK-803 induced myeloid differentiation of HL-60 cells. The active substance was identified as deamino-hydroxy-phoslactomycin B (HPLM) by mass spectrometry, and synthesized HPLM also induced the differentiation of HL-60 cells. HPLM showed greater inhibition of protein phosphatase 2A (PP2A) activity than phoslactomycin B (PLMB); however, PLMB and okadaic acid did not induce differentiation. Moreover, treatment with ATRA and 1α, 25(OH)2D3 induced retinoic acid receptor-β and 1α, 25(OH)2D3 24-hydroxylase, respectively, whereas HPLM did not, suggesting that HPLM is a novel differentiation inducer.
  • Simizu S, Teruya T, Nogawa T, Aono H, Ueki M, Uramoto M, Kobayashi Y, Osada H.: Deamino-hydroxy-phoslactomycin B, a biosynthetic precursor of phoslactomycin, induces myeloid differentiation in HL-60 cells.
    Biochem Biophys Res Commun, 383(4): 406-410 (2009) PMID: 19364501 [ doi: 10.1016/j.bbrc.2009.04.014 ]
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